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A simple, cost effective method for isolation and transportation of

Authors:

R L Ihalamulla,

Department of Parasitology, Faculty of Medicine, Kynsey Road, Colombo 8, LK
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U S Rajapaksa,

Department of Parasitology, Faculty of Medicine, Kynsey Road, Colombo 8, LK
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H V Y D Siriwardena,

Department of Parasitology, Faculty of Medicine, Kynsey Road, Colombo 8, LK
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M Chance,

The Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK., GB
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N D Karunaweera

Department of Parasitology, Faculty of Medicine, Kynsey Road, Colombo 8, LK
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Abstract

Isolation and propagation of Leishmania parasites using conventional culture methods are difficult, especially under field conditions. Transportation of live parasites requires the maintenance of low temperatures, which increases the cost considerably. The present study demonstrates that monophasic micro capillary culture is a simpler, more economical and better alternative to using conventional Evan's modified Tobie's medium to isolate and transport the Leishmania parasite over long distances without the use of temperature control methods.

Key words: cost effective, transportation, Leishmania, Sri Lanka)

Doi: 10.4038/cmj.v54i2.864

Ceylon Medical Journal Vol. 54, No. 2, June 2009 pp46-47

DOI: https://doi.org/10.4038/cmj.v54i2.864
How to Cite: Ihalamulla, R.L., Rajapaksa, U.S., Siriwardena, H.V.Y.D., Chance, M. and Karunaweera, N.D., 2009. A simple, cost effective method for isolation and transportation of. Ceylon Medical Journal, 54(2), pp.46–47. DOI: http://doi.org/10.4038/cmj.v54i2.864
Published on 24 Jul 2009.
Peer Reviewed

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