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STR polymorphisms in Sri Lanka: evaluation of forensic utility in identification of individuals and parentage testing

Authors:

Aresha Manamperi ,

Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, LK
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Chanditha Hapaurachchi,

Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, LK
About Chanditha
Department of Parasitology, Faculty of Medicine, University of Kelaniya, Thalagolla
Road, Ragama, Sri Lanka
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Nilmini Silva Gunawardene,

Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, LK
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Anura Bandara,

Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, LK
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Damsiri Dayanath,

Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, LK
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Wimaladharma Abeyewickreme

Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, LK
About Wimaladharma
Department of Parasitology, Faculty of Medicine, University of Kelaniya, Thalagolla
Road, Ragama, Sri Lanka
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Abstract

Objectives This preliminary study was carried out to determine the allele frequencies and forensic efficiency parameters for the short tandem repeat loci CSF1PO, TPOX, THO1, D16S539, D7S820, D13S317, vWA, FESFPS and F13B in a test sample population of Sri Lankans.
Design Test samples were obtained from 305 nonrelated individuals originating from all 9 provinces of Sri Lanka. DNA was extracted from whole blood using chelex- 100 and amplified by PCR using the GenePrint STR kit and silver stained. Final DNA profiles were analysed for forensic efficiency parameters and paternity indices using PowerStats version 12. Possible divergence from Hardy- Weinberg Equilibrium was tested using the chi-square test and exact test.
Results All common alleles in the allelic ladders were found in the test sample studied. PIC values >0.5 for all 9 STR loci indicate this STR system to be informative and useful for identification purposes. The D13S317, vWA and D7S820 loci were found to be the most polymorphic markers of the system studied.
Conclusions No deviations from Hardy-Weinberg Equilibrium were found for any of the loci examined. The results indicate that the 9 STR loci system described here is suitable for estimating DNA profile frequencies in human identification and forensic and parentage testing for legal purposes among Sri Lankans.

Key words: Population genetics, short tandem repeats, alleles, DNA fingerprinting, paternity testing, genetic database  

DOI: 10.4038/cmj.v54i3.1201

Ceylon Medical Journal Vol.54(3) 2009 85-89

DOI: https://doi.org/10.4038/cmj.v54i3.1201
How to Cite: Manamperi, A., Hapaurachchi, C., Gunawardene, N.S., Bandara, A., Dayanath, D. and Abeyewickreme, W., 2009. STR polymorphisms in Sri Lanka: evaluation of forensic utility in identification of individuals and parentage testing. Ceylon Medical Journal, 54(3), pp.85–89. DOI: http://doi.org/10.4038/cmj.v54i3.1201
Published on 13 Oct 2009.
Peer Reviewed

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