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Genotypes of hepatitis B virus identified in patients tested prior to endoscopy from a Teaching Hospital in the Central Province of Sri Lanka

Authors:

D.G.D. Jayasuriya ,

University of Peradeniya, LK
About D.G.D.
Department of Microbiology, Faculty of Medicine
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F Noordeen,

University of Peradeniya, LK
About F
Department of Microbiology, Faculty of Medicine
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F.N.N. Pitchai

University of Peradeniya, LK
About F.N.N.
Department of Microbiology, Faculty of Medicine
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Abstract

The present study was carried out to identify the hepatitis B virus (HBV) genotypes in six patients attending the surgical clinic who were positive for hepatitis B surface antigen. DNA was extracted from the serum of patients and subjected to a modified nested PCR to detect a 585 bp region within the S gene of the HBV genome. Positive PCR products were purified and sequenced via a cycle sequencing method. The sequence data were analyzed with reference sequences in the HepSEQ database to identify the particular HBV genotype of the samples. Nested PCR for the S gene of the HBV genome was positive in 2 out of 6 samples. The genotyping and sequence analysis of the PCR products showed HBV genotype A with a homology of 98% to the reference sequences in the HepSeq database.

Ceylon Medical Journal 2015; 60: 62-4

DOI: https://doi.org/10.4038/cmj.v60i2.8153
How to Cite: Jayasuriya, D.G.D., Noordeen, F. and Pitchai, F.N.N., 2015. Genotypes of hepatitis B virus identified in patients tested prior to endoscopy from a Teaching Hospital in the Central Province of Sri Lanka. Ceylon Medical Journal, 60(2), pp.62–64. DOI: http://doi.org/10.4038/cmj.v60i2.8153
Published on 14 Jun 2015.
Peer Reviewed

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