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Development and validation of an Enzyme Linked Immunosorbent Assay (ELISA) test for the diagnosis of toxoplasmosis in Sri Lanka

Authors:

D. Iddawela ,

Department of Parasitology, Faculty of Medicine, University of Peradeniya, LK
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K. Ehambaram,

Department of Parasitology, Faculty of Medicine, University of Peradeniya, LK
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P.V.R. Kumarasiri,

Department of Community Medicine, Faculty of Medicine, University of Peradeniya, LK
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S. Wijesundera

Department of Parasitology, Faculty of Medicine, University of Peradeniya, LK
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Abstract

Note: On 13 October 2015, on the website, the spelling of the second author was changed from 'Ehambaran' to 'Ehambaram' and the third author was changed from 'Kumarasir' to 'Kumarasiri'. The spellings were correct on the PDF.

Introduction ELISA is the most widely used form of diagnosis for toxoplasmosis. Several commercial kits are currently used in Sri Lanka. However, these kits are not affordable in resource-limited settings.

Objectives Aim of this study was to develop a cost effective in-house ELISA for the detection of Toxoplasma antibody and to estimate the diagnostic accuracy compared to a commercial kit.

Methods Vero cell lines were inoculated with tachyzoites and harvested after 2-6 days and sonicated to obtain somatic antigen. The antigen was used as coating material in ELISA to detect antibodies against T. gondii in patient sera. Hundred and three patients’ sera were analysed by in-house ELISA and kit ELISA. Optical density (OD) values were analysed statistically. Toxoplasma IgG avidity test was used to determine the chronic and acute phase of infection.

Results The optimum working dilutions for antigen was 0.846 μg/ml and for serum 1 in 100. The optimal cut-off values for the in-house ELISA within the range 0.85 to 0.98 at which the sensitivity was 95.3% and specificity was 98.3. The OD values of in-house ELISA were compared with OD values of kit ELISA and the results showed strong correlation between the two tests.

Conclusions The results of our study demonstrated that our in-house ELISA for detection of T. gondii antibody was as sensitive and specific as the commercial kit used in this study. Thus, the in-house ELISA is a useful, costeffective tool for diagnostic and screening purposes.

Ceylon Medical Journal 2015; 60: 82-86

How to Cite: Iddawela, D. et al., (2015). Development and validation of an Enzyme Linked Immunosorbent Assay (ELISA) test for the diagnosis of toxoplasmosis in Sri Lanka. Ceylon Medical Journal. 60(3), pp.82–86. DOI: http://doi.org/10.4038/cmj.v60i3.8185
Published on 27 Sep 2015.
Peer Reviewed

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