Original article
New forms of induction of apoptosis in aggressive lymphoma using peptides that interrupt the RAS / RAF interaction
Authors:
C. Bergna ,
National University of La Plata, AR
About C.
Faculty of Medical Science
G. H. Marin,
National University of La Plata, AR
About G. H.
Faculty of Medical Science
CONICET-UNLP
M. Maiz,
National University of La Plata, AR
About M.
Faculty of Medical Science
H. Bruzzoni Giovanelli,
Université Paris 7- Hôpital Saint Louis, FR
About H.
Pharmacology - Centre d’Investigations Cliniques
C. Ponzinibbio,
National University of La Plata, AR
About C.
Faculty of Medical Science
G. Schinella,
National University of La Plata, AR
About G.
Faculty of Medical Science
CIC- Scientific Research Commission- Buenos Aires
J. Errecalde,
National University of La Plata, AR
About J.
Faculty of Medical Science
A. Rebollo
CIMI, Inserm/UPMC/CNRS- Université Pierre et Marie Curie, Paris 6, FR
About A.
Centre d’Immunologie et des Maladies Infectieuses
Abstract
RAS-RAF-MEK-ERK is a key pathway for apoptosis regulation in cancer cells. B-Raf-inhibitors such as PLX4032 peptide was developed by Institute Curie-Université Pierre et Marie Curie in order to induce apoptosis in cancer cells.
OBJECTIVE
To demonstrate pro-apoptotic properties and survival outcome of EP2014/064243 peptide in murine aggressive lymphoma.
MATERIAL AND METHODS
BALBc mice with T-lymphoma were randomized assigned either in Group A (peptide+cyclophosphamide-CFM); Group B (peptides), Group C (CFM-control) or Control D (Cl-Na 0.9%-SF control group). Survival probability was calculated by Kaplan-Meier analysis. Apoptosis was detected using TUNEL technique. The protocol was approved by the Institutional Committee for Animal Care (CICUAL: T04-01-2015)
RESULTS
The median survival was 24 days (21.6-26.4) for placebo, 33 days (28.0-35.4) for the CFM monotherapy group, 33 (27.1-35.8) for the peptide group and 34 days (24,4-40) for CFM-peptide combined treatment (p<0.05). In lymph node tissue the mean TUNEL positive cells per field for each treatment group was 2, 12 and 13 and 35 for SF, CFM, peptide and combined therapy (p<0.05).
CONCLUSION
These findings suggest that in murine aggressive lymphoma treated by an experimental peptide in addition with CFM, had an exponentially pro-apoptotic effect than CFM alone, suggesting that the peptide potentiated the anti-tumoural effect of CFM.
How to Cite:
Bergna, C., Marin, G.H., Maiz, M., Bruzzoni Giovanelli, H., Ponzinibbio, C., Schinella, G., Errecalde, J. and Rebollo, A., 2019. New forms of induction of apoptosis in aggressive lymphoma using peptides that interrupt the RAS / RAF interaction. Ceylon Medical Journal, 64(2), pp.46–51. DOI: http://doi.org/10.4038/cmj.v64i2.8890
Published on
26 Jul 2019.
Peer Reviewed
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