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Screening for performance enhancing substances and quantification of ethanol in different Arishta manufactured in Sri Lanka

Authors:

Punchividanelage Nilu Jayashika Fernando ,

Sri Lanka Anti-Doping Agency, LK
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Shehani Pigera,

Sri Lanka Anti-Doping Agency, LK
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Suraweera Arachchilage Nimesha Rashani,

Sri Lanka Anti-Doping Agency, LK
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Ravindra Fernando,

National Dangerous Drugs Control Board, Rajagiriya, LK
About Ravindra

The National Narcotics Laboratory

 

Sir John Kotelawala Defence University, Ratmalana

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Pabasara Weerasinghe,

National Dangerous Drugs Control Board, LK
About Pabasara
The National Narcotics Laboratory
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Tharaka Deepal Godakumbura,

National Dangerous Drugs Control Board, LK
About Tharaka Deepal
The National Narcotics Laboratory
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Madunil Anuk Niriella,

University of Kelaniya, Dalugama, Kelaniya, LK
About Madunil Anuk
Faculty of Medicine
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Seevali Jayawickreme,

Sri Lanka Anti-Doping Agency, Colombo, LK
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Arjuna Priyadarshana de Silva

Sri Lanka Anti-Doping Agency, Colombo, LK
About Arjuna Priyadarshana
Faculty of Medicine, University of Kelaniya, Dalugama, Kelaniya
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Abstract

Background: Arishta have been used in Ayurveda medicine for over thousands of years in Sri Lanka to treat various diseases. Ashwagandharishta, Balarishta and Dashamoolarishta are usually prescribed to obtain an anabolic effect, and Ashwagandharishta and Dashamoolarishta for androgenic effect in males. Thus, these arishta have been shown to have similar effect as anabolic androgenic steroids and stimulants in Western medicine. Therefore, arishta could potentially be used by athletes to improve their performance in sports leading to unintentional doping. Additionally, ethanol develops in-source during arista fermentation, which can affect athletes health.


Objective: The aim of this study is to investigate whether the anabolic androgenic steroids or stimulants banned by World Anti-Doping Agency are present in these arishta,  and to determine their ethanol content.


Methods: Methanol extractions of Ashwagandarishta, Balarishta, Dashamoolarishta from four different manufacturers were screened for 21 stimulant and 22 anabolic androgenic steroids banned by World Anti-Doping Agency, using Gas Chromatography Mass Spectrometer. Ethanol content of the twelve Arishta samples were also measured.


Results: Anabolic androgenic steroids or stimulants were not present in the tested Arishta samples, and percentage volume / volume ethanol content of all Arishta was between (5.80–8.35) ±0.5.


Conclusion: The tested brands of Ashwagandharishta, Balarishta and Dashamoolarishta did not contain stimulants or anabolic androgenic steroids banned by World Anti-Doping Agency.

DOI: https://doi.org/10.4038/cmj.v65i4.9282
How to Cite: Jayashika Fernando, P.N., Pigera, S., Nimesha Rashani, S.A., Fernando, R., Weerasinghe, P., Godakumbura, T.D., Niriella, M.A., Jayawickreme, S. and de Silva, A.P., 2020. Screening for performance enhancing substances and quantification of ethanol in different Arishta manufactured in Sri Lanka. Ceylon Medical Journal, 65(4), pp.112–117. DOI: http://doi.org/10.4038/cmj.v65i4.9282
Published on 31 Dec 2020.
Peer Reviewed

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